rabbit polyclonal total ampk (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
rabbit polyclonal total ampk
Rabbit Polyclonal Total Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 4496 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+total+ampk/AMPKalpha+Antibody/pm41373646-180-18-24
Average 97 stars, based on 4496 article reviews
Rabbit Polyclonal Total Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 4496 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+total+ampk/AMPKalpha+Antibody/pm41373646-180-18-24
Average 97 stars, based on 4496 article reviews
rabbit polyclonal total ampk - by Bioz Stars,
2026-09
97/100 stars
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Blocking Assay:Article Title: Pharmacological inhibition of casein kinase II attenuates metaflammation in a murine model of diet-induced metabolic dysfunction Article Snippet: Briefly, 50 μ g proteins were separated by SDS-PAGE on 8, 10 and 12% acrylamide gels, and were transferred to polyvinylidene difluoride membranes (cat. no. GE10600038; Merck KGaA) or nitrocellulose membranes (cat. no. GVS1215471; GVS S.p.a.). .. After 1 h of blocking with 10% BSA solution, the membranes were incubated at 4°C overnight with the following primary antibodies: Rabbit anti-phosphorylated (p)-CK2α pTyr 255 (1:1,000; cat. no. SAB4504299; Merck KGaA) rabbit anti-total CK2α (1:1,000; cat. no. ab76040; Abcam), mouse anti-Ser 32/36 IκBα (1:1,000, cat. no. 9246), rabbit anti-total IκBα (1:1,000; cat. no. 9242), rabbit anti-total p65 NFκB (1:500; cat. no. 8242) (all from Cell Signaling Technology, Inc.), mouse anti-nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 (NLRP3; 1:1,000; cat. no. AG-20B-0014-C100; AdipoGen Life Sciences), rabbit anti-cleaved caspase-1 (1:500; cat. no. 89332; Cell Signaling Technology, Inc.), mouse anti-total caspase 1 (1:1,000; cat. no. MA5-16215; Thermo Fisher Scientific, Inc.), rabbit anti-p-AMPK (1:1,000; cat. no. 2531), Incubation:Article Title: Pharmacological inhibition of casein kinase II attenuates metaflammation in a murine model of diet-induced metabolic dysfunction Article Snippet: Briefly, 50 μ g proteins were separated by SDS-PAGE on 8, 10 and 12% acrylamide gels, and were transferred to polyvinylidene difluoride membranes (cat. no. GE10600038; Merck KGaA) or nitrocellulose membranes (cat. no. GVS1215471; GVS S.p.a.). .. After 1 h of blocking with 10% BSA solution, the membranes were incubated at 4°C overnight with the following primary antibodies: Rabbit anti-phosphorylated (p)-CK2α pTyr 255 (1:1,000; cat. no. SAB4504299; Merck KGaA) rabbit anti-total CK2α (1:1,000; cat. no. ab76040; Abcam), mouse anti-Ser 32/36 IκBα (1:1,000, cat. no. 9246), rabbit anti-total IκBα (1:1,000; cat. no. 9242), rabbit anti-total p65 NFκB (1:500; cat. no. 8242) (all from Cell Signaling Technology, Inc.), mouse anti-nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 (NLRP3; 1:1,000; cat. no. AG-20B-0014-C100; AdipoGen Life Sciences), rabbit anti-cleaved caspase-1 (1:500; cat. no. 89332; Cell Signaling Technology, Inc.), mouse anti-total caspase 1 (1:1,000; cat. no. MA5-16215; Thermo Fisher Scientific, Inc.), rabbit anti-p-AMPK (1:1,000; cat. no. 2531), Article Title: Impaired Autophagy Flux is Associated with Proinflammatory Microglia Activation Following Japanese Encephalitis Virus Infection. Article Snippet: Role of autophagy in Japanese encephalitis viral (JEV) infection is not well known.. In the present study, we reported the role of autophagy flux in microglia activation, neurobehavioral function and neuronal death using a mouse model of JEV.. Markers for autophagy (LC3-II/I, SQSTM1/P62, phos-Akt, phos-AMPK), and neuronal death (cleaved caspase 12, H2Ax, polyubiquitin) were investigated by western blot at 1, 3 and 7 days post inoculation. Cell Culture:Article Title: GKRP-dependent modulation of feeding behavior by tanycyte-released monocarboxylates Article Snippet: .. Small pieces of tissue or cultured primary cells were homogenized in RIPA buffer supplemented with complete protease and phosphatase inhibitor cocktail (ThermoFisher Scientific) and then sonicated three times on ice at 300 W (Sonics & Material INC, VCF1, Connecticut, USA) for 10 s. After isolation by centrifugation at 8,000× g for 10 min, the proteins were quantified and resolved by SDS-PAGE (50 μg/lane) in a 10% (w/v) polyacrylamide gel, transferred to PVDF membranes (0.45 μm pore, Amersham Pharmacia Biotech., Piscataway, NJ, USA), and probed with mouse anti-GK (1:2000, Santa Cruz Biotechnology, CA, USA), rabbit anti-GKRP (1:1000, Santa Cruz Biotechnology), rabbit anti-GLUT2 (1:1000, Alpha Diagnostics), mouse anti-EGFP (1:2000, Santa Cruz Biotechnology), rabbit anti-MCT1 (1:1000, Millipore), Sonication:Article Title: GKRP-dependent modulation of feeding behavior by tanycyte-released monocarboxylates Article Snippet: .. Small pieces of tissue or cultured primary cells were homogenized in RIPA buffer supplemented with complete protease and phosphatase inhibitor cocktail (ThermoFisher Scientific) and then sonicated three times on ice at 300 W (Sonics & Material INC, VCF1, Connecticut, USA) for 10 s. After isolation by centrifugation at 8,000× g for 10 min, the proteins were quantified and resolved by SDS-PAGE (50 μg/lane) in a 10% (w/v) polyacrylamide gel, transferred to PVDF membranes (0.45 μm pore, Amersham Pharmacia Biotech., Piscataway, NJ, USA), and probed with mouse anti-GK (1:2000, Santa Cruz Biotechnology, CA, USA), rabbit anti-GKRP (1:1000, Santa Cruz Biotechnology), rabbit anti-GLUT2 (1:1000, Alpha Diagnostics), mouse anti-EGFP (1:2000, Santa Cruz Biotechnology), rabbit anti-MCT1 (1:1000, Millipore), Isolation:Article Title: GKRP-dependent modulation of feeding behavior by tanycyte-released monocarboxylates Article Snippet: .. Small pieces of tissue or cultured primary cells were homogenized in RIPA buffer supplemented with complete protease and phosphatase inhibitor cocktail (ThermoFisher Scientific) and then sonicated three times on ice at 300 W (Sonics & Material INC, VCF1, Connecticut, USA) for 10 s. After isolation by centrifugation at 8,000× g for 10 min, the proteins were quantified and resolved by SDS-PAGE (50 μg/lane) in a 10% (w/v) polyacrylamide gel, transferred to PVDF membranes (0.45 μm pore, Amersham Pharmacia Biotech., Piscataway, NJ, USA), and probed with mouse anti-GK (1:2000, Santa Cruz Biotechnology, CA, USA), rabbit anti-GKRP (1:1000, Santa Cruz Biotechnology), rabbit anti-GLUT2 (1:1000, Alpha Diagnostics), mouse anti-EGFP (1:2000, Santa Cruz Biotechnology), rabbit anti-MCT1 (1:1000, Millipore), Centrifugation:Article Title: GKRP-dependent modulation of feeding behavior by tanycyte-released monocarboxylates Article Snippet: .. Small pieces of tissue or cultured primary cells were homogenized in RIPA buffer supplemented with complete protease and phosphatase inhibitor cocktail (ThermoFisher Scientific) and then sonicated three times on ice at 300 W (Sonics & Material INC, VCF1, Connecticut, USA) for 10 s. After isolation by centrifugation at 8,000× g for 10 min, the proteins were quantified and resolved by SDS-PAGE (50 μg/lane) in a 10% (w/v) polyacrylamide gel, transferred to PVDF membranes (0.45 μm pore, Amersham Pharmacia Biotech., Piscataway, NJ, USA), and probed with mouse anti-GK (1:2000, Santa Cruz Biotechnology, CA, USA), rabbit anti-GKRP (1:1000, Santa Cruz Biotechnology), rabbit anti-GLUT2 (1:1000, Alpha Diagnostics), mouse anti-EGFP (1:2000, Santa Cruz Biotechnology), rabbit anti-MCT1 (1:1000, Millipore), SDS Page:Article Title: GKRP-dependent modulation of feeding behavior by tanycyte-released monocarboxylates Article Snippet: .. Small pieces of tissue or cultured primary cells were homogenized in RIPA buffer supplemented with complete protease and phosphatase inhibitor cocktail (ThermoFisher Scientific) and then sonicated three times on ice at 300 W (Sonics & Material INC, VCF1, Connecticut, USA) for 10 s. After isolation by centrifugation at 8,000× g for 10 min, the proteins were quantified and resolved by SDS-PAGE (50 μg/lane) in a 10% (w/v) polyacrylamide gel, transferred to PVDF membranes (0.45 μm pore, Amersham Pharmacia Biotech., Piscataway, NJ, USA), and probed with mouse anti-GK (1:2000, Santa Cruz Biotechnology, CA, USA), rabbit anti-GKRP (1:1000, Santa Cruz Biotechnology), rabbit anti-GLUT2 (1:1000, Alpha Diagnostics), mouse anti-EGFP (1:2000, Santa Cruz Biotechnology), rabbit anti-MCT1 (1:1000, Millipore), Membrane:Article Title: Impaired Autophagy Flux is Associated with Proinflammatory Microglia Activation Following Japanese Encephalitis Virus Infection. Article Snippet: Role of autophagy in Japanese encephalitis viral (JEV) infection is not well known.. In the present study, we reported the role of autophagy flux in microglia activation, neurobehavioral function and neuronal death using a mouse model of JEV.. Markers for autophagy (LC3-II/I, SQSTM1/P62, phos-Akt, phos-AMPK), and neuronal death (cleaved caspase 12, H2Ax, polyubiquitin) were investigated by western blot at 1, 3 and 7 days post inoculation. |
